علوم زیستی دریا
N Nozarpour; M.B Nabavi; Ronagh M.T Ronagh; B Archangi; N Sakhai
Abstract
In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to ...
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In this study, the molecular characteristics of Chiromantes boulengeri in 2014 from intertidal waters of Arvand River in the vicinity of Minoo island were investigated. So, in October 2014 specimens from intertidal of two area of Minoo island were collected and preserved in 70% ethanol, carried out to the khoramshahr University of Marine Science and Technology laboratory and examined. To fulfill the purpose, To extract the DNA, phenol- chloroform method were used and mitochondrial gene 16S rRNA was sequenced and compare with other species from GenBank NCBI. The morphological and phylogeny sequencing results showed that all specimens belong to Chiromantes boulengeri in the study area. By polymorphism analysis, there are mutation within C. boulengeri from two area of Arvand River and it seems that this species for its survival has adapted with different ecological conditions in the study area.
علوم زیستی دریا
Esmat Salimi; hossein zolgharnein; Bita Archangi; Mohammad Taghi Ronagh; Seyyed Ahmad Ghasemi
Abstract
In this study, two populations of Aphanius ginaonis (Holly, 1929) and Aphanius dispar (Ruppell, 1828) were examined to determine genetic diversity using PCR-RFLP technique. A total of 60 individual specimens were collected from Hormozgan and Bushehr internal waters, comprising two sampling sites; from ...
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In this study, two populations of Aphanius ginaonis (Holly, 1929) and Aphanius dispar (Ruppell, 1828) were examined to determine genetic diversity using PCR-RFLP technique. A total of 60 individual specimens were collected from Hormozgan and Bushehr internal waters, comprising two sampling sites; from Geno hot spring (30 individuals of A. ginaonis) and Mir Ahmad hot spring (30 individuals of A. dispar). The D-loop region of mitochondrial DNA (550 bp) was amplified using PCR followed by RFLP analysis based on 5 restriction endonucleas enzymes (AluI, DpnI, Eco47I, HindIII HinfI). In order to undertake data analysis, Arlequin 3.11 was applied. Results at population levels indicated that Geno individuals have more haplotype diversity than Mir Ahmad individuals (9 and 3 haplotypes respectively). However, further investigation using genetic techniques is required to clarify the molecular history and evolution of Aphanius species in this area. Results obtained from this research would be applicable to understand conservation genetics and management of this important fish species in Iran.
Alireaza Safahieh; Sahel Pakzadtoochaei; Mohammad Taghi Ronagh; Bita Archangi; Mohammad Ali Hamzeh
Volume 12, Issue 3 , September 2014, , Pages 15-25
Abstract
Heavy metals in marine ecosystems are from both natural and anthropogenic sources. The monsoon is a natural phenomenon that can effect the heavy metals concentration. The monsoon can affect heavy metal concentration in coastal areas through rainfall and leaching and also disrupting of water layer. The ...
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Heavy metals in marine ecosystems are from both natural and anthropogenic sources. The monsoon is a natural phenomenon that can effect the heavy metals concentration. The monsoon can affect heavy metal concentration in coastal areas through rainfall and leaching and also disrupting of water layer. The effect of monsoon on changes of concentrations heavy metals such as Cd, Cu, Ni, Pb and Zn in the sediment of Chabahar coast were studied in 2010. Samples were collected from the sediment of Gwatr, Beris, Ramin, Chabahar, Tis and Pozm stations in premonsoon, monsoon and post monsoon seasons (May, August and November respectively) during low tide. After preparation of samples, heavy metal concentration was determined by using the atomic absorption spectrophotometer. The concentration of Cd, Cu, Ni, Pb and Zn were 0.36, 4.97, 17.14, 8.88 and 24.93 µg/g dw respectively. The Cd concentration in the sediment was influenced by regional sources. The increase of Cu and Zn concentration from monsoon until post monsoon could be caused by rainfall and disturbance in the coastal sediments. The increase of Pb concentration in monsoon and post monsoon could be due to rapid precipitations of Pb, this increase could be affected due to coastal runoff in monsoon and the resumption of the vessel’s activity in post monsoon.
Ahmad Shadi; Hosein Zolgharnein; MohamadAli Salari-aliabadi; Mohamad Bagher Nabavi; Mohamad Taghi Ronagh
Abstract
Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 ...
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Using 5 microsatellite loci Sillago sp. From Hormozgan North Persian Gulf were investigated through 68 specimens in two stations: Minab(31 specimens) and Bandar Lengeh(38 specimens). DNA was extracted using modified CTAB(Hexadecyl trimethyl-ammonium bromide) protocol. 5-17 alleles were observed in 5 loci with 8.4 mean allelic frequency. Observed and expected heterozygosity values was calculated (0.115<Ho<0.792) ,(0.598<He<0.902) . Genetic distance and identity according to Nei was estimated 0.520 and 0.595 respectively which shows genetic distance of genus level. Significant deviation from Hardy-Weinberg equilibrium (p<0.001) was observed at all loci at two stations. Analysis of Molecular Variance(AMOVA) of Genetic differentiation index(Fst) revealed mean significant (p=0.001) differentiation between two studied regions and 3.90 gen flow. Despite low distance of two studied regions (180km) there are probably two distinct populations of Sillago sp. which should be considered in management efforts.